Architecture and subunit dynamics of the mitochondrial TIM9·10·12 chaperone

Weinhäupl K, Wang Y, Hessel A, Brennich M, Lindorff-Larsen K, Schanda P
(2020 Mar 14)

doi: 10.1101/2020.03.13.990150 		Submitted to SASBDB: 2020 Mar 10
Published in SASBDB:

SASDH28 – Mitochondrial import inner membrane translocase TIM 9·10·12

Mitochondrial import inner membrane translocase subunit TIM12Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM12 monomer, 12 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM9 dimer, 20 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM MES buffer (2-(N-morpholino)ethanesulfonic acid), pH 6.5, 50 mM NaCl Cloning,, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 8
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3