Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ₁ RNA Riboswitches.

Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
J Mol Biol 432(16):4523-4543 (2020 Jul 24)

PMID: 32522558 doi: 10.1016/j.jmb.2020.06.002

Submitted to SASBDB: 2020 Mar 8 Published in SASBDB: 2020 Mar 14

SASDH38 – Wildtype preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-WT-apo Sample: Wildtype preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 2.6 nm Dmax 10.5 nm VolumePorod 20 nm3

SASDH48 – Wildtype preQ1 riboswitch in Bacillus subtilis, holo-state - Bsu-WT-holo Sample: Wildtype preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 2.0 nm Dmax 9.0 nm VolumePorod 16 nm3

SASDH58 – C15 deletion preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-C15d-apo Sample: C15 deletion preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.9 nm Dmax 8.0 nm VolumePorod 17 nm3

SASDH68 – C15 deletion preQ1 riboswitch in Bacillus subtilis, holo-state - Bsu-C15d-holo Sample: C15 deletion preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 2.0 nm Dmax 8.8 nm VolumePorod 14 nm3

SASDH78 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state - Tte-WT-apo Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.6 nm Dmax 6.7 nm VolumePorod 12 nm3

SASDH88 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state - Tte-WT-holo Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.6 nm Dmax 7.2 nm VolumePorod 13 nm3

SASDHB8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state - Tte-A14'-apo Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.9 nm Dmax 9.0 nm VolumePorod 14 nm3

SASDHC8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state - Tte-A14'-holo Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.8 nm Dmax 12.0 nm VolumePorod 16 nm3

SASDH98 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state high-temperature sampling - Tte-WT-apo-HT Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.7 nm Dmax 7.0 nm VolumePorod 11 nm3

SASDHA8 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state high-temperature sampling - Tte-WT-holo-HT Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.5 nm Dmax 6.5 nm VolumePorod 10 nm3

SASDHD8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state high-temperature sampling - Tte-A14'-apo-HT Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.8 nm Dmax 7.5 nm VolumePorod 13 nm3

SASDHE8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state high-temperature sampling - Tte-A14'-holo-HT Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23 RgGuinier 1.9 nm Dmax 8.8 nm VolumePorod 14 nm3