Structural basis of client specificity in mitochondrial membrane-protein chaperones.

Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P
Sci Adv 6(51) (2020 Dec)

PMID: 33355130 doi: 10.1126/sciadv.abd0263

Submitted to SASBDB: 2020 Jun 8 Published in SASBDB: 2020 Jun 17

SASDH89 – Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 24 RgGuinier 3.1 nm Dmax 11.3 nm

SASDJP4 – Mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23 Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2017 May 18 RgGuinier 3.2 nm Dmax 7.7 nm VolumePorod 146 nm3

SASDJQ4 – Mitochondrial import inner membrane translocase subunits TIM8-TIM13 Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 3.1 nm Dmax 10.9 nm VolumePorod 136 nm3