A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex.

Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
J Mol Biol 428(8):1589-603 (2016 Apr 24)

PMID: 26996937 doi: 10.1016/j.jmb.2016.03.007

Submitted to SASBDB: 2015 Aug 24 Published in SASBDB: 2016 Mar 18

SASDAA9 – EcPaaA2-EcParE2His construct Sample: Plasmid stabilization protein ParE 16-mer, 188 kDa Escherichia coli protein Uncharacterized protein (Antitoxin) 16-mer, 135 kDa Escherichia coli protein Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 9 RgGuinier 3.8 nm Dmax 16.2 nm VolumePorod 312 nm3

SASDAB9 – EcPaaA2-HisEcParE2 construct Sample: Plasmid stabilization protein ParE octamer, 102 kDa Escherichia coli protein Uncharacterized protein (Antitoxin) octamer, 68 kDa Escherichia coli protein Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5 RgGuinier 3.3 nm Dmax 15.3 nm VolumePorod 166 nm3

SASDC84 – parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Sample: Plasmid stabilization protein ParE monomer, 13 kDa Escherichia coli protein Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5 RgGuinier 2.2 nm Dmax 9.3 nm VolumePorod 36 nm3