Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility

Orr C, Fisher H, Yu X, Chan C, Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
Science Immunology 7(73) (2022 Jul 08)

doi: 10.1126/sciimmunol.abm3723

Submitted to SASBDB: 2021 Apr 16 Published in SASBDB: 2022 Jul 11

SASDLF4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant Sample: ChiLob 7/4 IgG2 F(ab)2 C224S mutant monomer, 99 kDa protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9 RgGuinier 3.9 nm Dmax 14.2 nm VolumePorod 135 nm3

SASDLG4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant Sample: ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant monomer, 99 kDa protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9 RgGuinier 4.2 nm Dmax 15.1 nm VolumePorod 135 nm3

SASDLH4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant Sample: ChiLob 7/4 IgG2 F(ab)2 C225S mutant monomer, 99 kDa protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9 RgGuinier 4.0 nm Dmax 14.5 nm VolumePorod 131 nm3

SASDLJ4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant Sample: ChiLob 7/4 IgG2 F(ab)2 C228S mutant monomer, 99 kDa protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9 RgGuinier 4.1 nm Dmax 13.5 nm VolumePorod 135 nm3

SASDLK4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant Sample: ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant monomer, 99 kDa protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9 RgGuinier 4.0 nm Dmax 13.3 nm VolumePorod 133 nm3

SASDLL4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant Sample: ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant monomer, 99 kDa protein Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9 RgGuinier 4.0 nm Dmax 13.7 nm VolumePorod 130 nm3