Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis.

Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
Biochemistry (2021 Jan 8)
PMID: 33416314
doi: 10.1021/acs.biochem.0c00830 		Submitted to SASBDB: 2020 Oct 8
Published in SASBDB:

SASDJ49 – Apo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Apo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 83 nm3

SASDJ59 – Holo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Holo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 72 nm3