The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity andcontributes to membrane binding.

Ravala SK, Hopkins JB, Plescia CB, Allgood SR, Kane MA, Cash JN, Stahelin RV, Tesmer JJG
J Biol Chem (2020 Jul 13)

PMID: 32661198 doi: 10.1074/jbc.RA120.014534

Submitted to SASBDB: 2020 Apr 10 Published in SASBDB: 2020 Jul 17

SASDHY9 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-408): DHPH Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein monomer, 43 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300mM NaCl, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19 RgGuinier 2.8 nm Dmax 10.5 nm VolumePorod 64 nm3

SASDHW9 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): DHPHDEP1 Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein monomer, 54 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19 RgGuinier 3.0 nm Dmax 10.4 nm VolumePorod 77 nm3

SASDHX9 – Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1 Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 monomer, 54 kDa Homo sapiens protein Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19 RgGuinier 3.0 nm Dmax 10.5 nm VolumePorod 75 nm3