Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars

Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
ACS Catalysis (2020 Jul 22)

doi: 10.1021/acscatal.0c02117

Submitted to SASBDB: 2020 Jun 2 Published in SASBDB: 2020 Jul 23

SASDHG9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - apo form Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT,, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27 RgGuinier 2.5 nm Dmax 8.8 nm VolumePorod 77 nm3

SASDHH9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27 RgGuinier 2.4 nm Dmax 8.5 nm VolumePorod 76 nm3

SASDHJ9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and trehalose Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27 RgGuinier 2.4 nm Dmax 8.3 nm VolumePorod 76 nm3

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27 RgGuinier 2.4 nm Dmax 8.5 nm VolumePorod 73 nm3