Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding

Karen Manalastas-Cantos Submitted to SASBDB: 2020 Apr 15
Published in SASBDB:

SASDHZ9 – Condensin complex subunit 1, Ycs4

Condensin complex subunit 1 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
RgGuinier 5.2 nm
Dmax 17.7 nm
VolumePorod 296 nm3

SASDJ22 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 336-418 (Brn1S)

Condensin complex subunit 1Condensin complex subunit 2, 336-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 336-418 monomer, 9 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
RgGuinier 5.0 nm
Dmax 16.9 nm
VolumePorod 279 nm3

SASDJ32 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 225-418 (Brn1L)

Condensin complex subunit 1Condensin complex subunit 2, 225-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
RgGuinier 4.9 nm
Dmax 15.9 nm
VolumePorod 309 nm3

SASDJ42 – Pentameric assembly of condensin complex subunits 1 and 2 (Ycs4-Brn1L-Brn1C) with subunits of the SMC hinge domain-containing protein (Smc4hd)

Condensin complex subunit 1Condensin complex subunit 2, 225-418Condensin complex subunit 2, 776-898SMC hinge domain-containing protein, 263-466SMC hinge domain-containing protein, 1367-1542 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 776-898 monomer, 14 kDa Chaetomium thermophilum protein
SMC hinge domain-containing protein, 263-466 monomer, 22 kDa Chaetomium thermophilum protein
SMC hinge domain-containing protein, 1367-1542 monomer, 20 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
RgGuinier 5.1 nm
Dmax 17.9 nm
VolumePorod 355 nm3