Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.

Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V
Nucleic Acids Res 45(6):3547-3558 (2017 Apr 7)

PMID: 28039325 doi: 10.1093/nar/gkw1310

Submitted to SASBDB: 2020 Apr 14 Published in SASBDB: 2020 Apr 20

SASDJB2 – Restriction endonuclease R.AgeI in apo form, monomeric Sample: Type-2 restriction enzyme AgeI monomer, 31 kDa Thalassobius gelatinovorus protein Buffer: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14 RgGuinier 2.2 nm Dmax 6.7 nm VolumePorod 53 nm3

SASDJC2 – Restriction endonuclease R.AgeI complex with cognate DNA Sample: Type-2 restriction enzyme AgeI dimer, 61 kDa Thalassobius gelatinovorus protein Cognate DNA oligoduplex with 5'-T overhang dimer, 8 kDa DNA Buffer: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14 RgGuinier 2.4 nm Dmax 7.4 nm VolumePorod 69 nm3