An atypical BRCT-BRCT interaction with the XRCC1 scaffold protein compacts human DNA Ligase IIIα within a flexible DNA repair complex.

Hammel M, Rashid I, Sverzhinsky A, Pourfarjam Y, Tsai MS, Ellenberger T, Pascal JM, Kim IK, Tainer JA, Tomkinson AE
Nucleic Acids Res (2020 Dec 16)

PMID: 33330937 doi: 10.1093/nar/gkaa1188

Submitted to SASBDB: 2020 Apr 15 Published in SASBDB: 2020 Apr 16

SASDJ52 – DNA repair protein XRCC1ΔN monomer/dimer Sample: DNA repair protein XRCC1ΔN dimer, 76 kDa Homo sapiens protein Buffer: 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2011 Jul 15 RgGuinier 5.4 nm Dmax 19.5 nm VolumePorod 170 nm3

SASDJ62 – DNA repair protein XRCC1 monomer /dimer Sample: DNA repair protein XRCC1, 69 kDa Homo sapiens protein Buffer: 200 mM NaCl, 20 mM Tris-HCl, pH 7.5, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 19 RgGuinier 6.3 nm Dmax 26.9 nm VolumePorod 330 nm3

SASDJ72 – DNA Ligase IIIα monomer/dimer Sample: DNA ligase 3 (DNA ligase III alpha), Homo sapiens protein Buffer: 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2011 Mar 31 RgGuinier 6.1 nm Dmax 21.0 nm VolumePorod 240 nm3

SASDJ82 – DNA repair protein XRCC1 - DNA Ligase IIIα complex Sample: DNA repair protein XRCC1, 69 kDa Homo sapiens protein DNA ligase 3 (DNA ligase III alpha), Homo sapiens protein Buffer: 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl₂,  0.2 mM PMSF, 1 mM benzamidine, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Aug 18 RgGuinier 6.2 nm Dmax 27.9 nm VolumePorod 675 nm3