PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum.

Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
Int J Parasitol (2020 Sep 5)

PMID: 32896572 doi: 10.1016/j.ijpara.2020.07.010

Submitted to SASBDB: 2020 Apr 28 Published in SASBDB: 2020 Oct 30

SASDJT2 – DNA-directed RNA polymerase (RPO41) Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Sep 25 RgGuinier 4.4 nm Dmax 12.5 nm VolumePorod 295 nm3

SASDJU2 – DNA-directed RNA polymerase (RPO41) in complex with rRNA adenine N(6)-methyltransferase (PfKsgA1) Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein RRNA adenine N (6) methyl transferase monomer, 39 kDa Plasmodium falciparum protein Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Jan 10 RgGuinier 5.5 nm Dmax 15.0 nm VolumePorod 393 nm3

SASDJV2 – DNA-directed RNA polymerase (RPO41) in complex with mitochondrial transcription factor 1 (MTF1) Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein Mitochondrial transcription factor 1 monomer, 40 kDa Saccharomyces cerevisiae protein Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 7 RgGuinier 4.5 nm Dmax 14.4 nm VolumePorod 292 nm3