Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties

Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
Protein Science (2020 Dec 05)

doi: 10.1002/pro.4009

Submitted to SASBDB: 2020 Jun 2 Published in SASBDB: 2020 Dec 18

SASDJY3 – Immunoglobulin HUI-018 Fab in complex with human insulin Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein HUI-018 Fab monomer, 47 kDa Mus musculus protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5 Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7 RgGuinier 3.4 nm Dmax 10.7 nm VolumePorod 86 nm3

SASDJZ3 – Immunoglobulin HUI-018 Fab in complex with human insulin at high concentration Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein HUI-018 Fab monomer, 47 kDa Mus musculus protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5 Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7 RgGuinier 3.7 nm Dmax 13.4 nm VolumePorod 105 nm3

SASDJ24 – Immunoglobulin HUI-018 Fab and OXI-005 Fab in a ternary complex with porcine insulin Sample: HUI-018 Fab monomer, 47 kDa Mus musculus protein Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Sus scrofa protein OXI-005 Fab monomer, 47 kDa Mus musculus protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2019 Mar 28 RgGuinier 4.5 nm Dmax 14.2 nm VolumePorod 138 nm3