Interdomain Flexibility within NADPH Oxidase Suggested by SANS Using LMNG Stealth Carrier.

Vermot A, Petit-Härtlein I, Breyton C, Le Roy A, Thépaut M, Vivès C, Moulin M, Härtlein M, Grudinin S, Smith SME, Ebel C, Martel A, Fieschi F
Biophys J 119(3):605-618 (2020 Aug 4)
PMID: 32668232
doi: 10.1016/j.bpj.2020.06.025 		Submitted to SASBDB: 2020 May 18
Published in SASBDB:

SASDJ63 – Streptococcus pneumoniae NADPH oxidase - Tag-free SpNOX

FAD-binding FR-type domain-containing protein experimental SAS data
OTHER model
Sample: FAD-binding FR-type domain-containing protein monomer, 47 kDa Streptococcus pneumoniae protein
Buffer: 50 mM Tris-HCl pH 7, 300 mM NaCl, 5 mM LMNG, 10 µM FAD, 21.4% D₂O, pH: 7
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 26
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 89 nm3