Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2.

Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
Mol Cell (2021 Sep 10)

PMID: 34529927 doi: 10.1016/j.molcel.2021.08.024

Submitted to SASBDB: 2020 May 28 Published in SASBDB: 2021 Oct 1

SASDJ93 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type apo-form Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23 RgGuinier 2.1 nm Dmax 6.5 nm VolumePorod 45 nm3

SASDJA3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type bound to mono-ubiquitin Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein Polyubiquitin-C monomer, 9 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23 RgGuinier 2.3 nm Dmax 6.8 nm VolumePorod 58 nm3

SASDJB3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to di-ubiquitin Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23 RgGuinier 2.5 nm Dmax 8.0 nm VolumePorod 68 nm3

SASDJC3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tri-ubiquitin Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C trimer, 26 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26 RgGuinier 2.8 nm Dmax 8.8 nm VolumePorod 97 nm3

SASDJD3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tetra-ubiquitin Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C tetramer, 34 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26 RgGuinier 2.9 nm Dmax 9.2 nm VolumePorod 111 nm3

SASDJE3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to penta-ubiquitin Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C pentamer, 43 kDa Homo sapiens protein Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26 RgGuinier 2.8 nm Dmax 9.0 nm VolumePorod 116 nm3