Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples.

Castellví A, Pascual-Izarra C, Crosas E, Malfois M, Juanhuix J
Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020 Oct 1)

PMID: 33021499 doi: 10.1107/S2059798320010700

Submitted to SASBDB: 2020 May 28 Published in SASBDB: 2020 Oct 30

SASDJF3 – Aldo-keto reductase family 1 member B1 (AKR1B1) in the presence of 100 mM 5-methyl uridine Sample: Aldo-keto reductase family 1 member B1 monomer, 36 kDa Homo sapiens protein Buffer: 20 mM Tris, 250 mM NaCl, 100 mM 5-methyl uridine, pH: 8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14 RgGuinier 2.0 nm Dmax 6.5 nm VolumePorod 52 nm3

SASDJG3 – Bovine Liver Catalase in presence of 100 mM 5-methyl uridine Sample: Catalase tetramer, 240 kDa Bos taurus protein Buffer: 50 mM sodium phosphate, pH: 7 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14 RgGuinier 3.7 nm Dmax 11.5 nm VolumePorod 265 nm3

SASDJH3 – Hen Egg White Lysozyme in presence of 100 mM 5-methyl uridine Sample: Lysozyme C monomer, 16 kDa Gallus gallus protein Buffer: 40 mM sodium acetate, 150 mM NaCl, 100 mM 5-methyl uridine, pH: 3.8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 7 RgGuinier 1.5 nm Dmax 4.2 nm VolumePorod 21 nm3

SASDJJ3 – SH3 domain of alpha spectrin (amino acids 965-1025) in presence of 100 mM 5-methyl uridine Sample: Spectrin alpha chain, non-erythrocytic 1, 7 kDa Gallus gallus protein Buffer: 10 mM citric acid, 150 mM NaCl, 100 mM 5-methyl uridine, pH: 3 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14 RgGuinier 1.3 nm Dmax 4.5 nm VolumePorod 10 nm3