Solution structure and dynamics of the mitochondrial‐targeted GTPase ‐activating protein ( GAP ) VopE by an integrated NMR / SAXS approach

Smith K, Lee W, Tonelli M, Lee Y, Light S, Cornilescu G, Chakravarthy S
Protein Science (2022 Feb 08)

doi: 10.1002/pro.4282
Submitted to SASBDB: 2020 Jun 4
Published in SASBDB:

SASDJV3 – GTPase-Activation Protein (GAP) domain (73-204) of Vibrio cholerae Outer Protein E (VopE)

Outer membrane virulence protein yopE experimental SAS data
Outer membrane virulence protein yopE Kratky plot
Sample: Outer membrane virulence protein yopE monomer, 15 kDa Vibrio cholerae protein
Buffer: 10 mM HEPES-HCl, pH 7.4, 150 mM NaCl, 0.5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Jun 29
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3