A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells

Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
Advanced Science :2003630 (2021 Mar 08)

doi: 10.1002/advs.202003630 		Submitted to SASBDB: 2020 Jun 5
Published in SASBDB:

SASDJ64 – Calcium bound Calmodulin

Calmodulin-1 experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 27 nm3

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3