Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly

Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
iScience 24(10):103153 (2021 Oct)

doi: 10.1016/j.isci.2021.103153

Submitted to SASBDB: 2020 Jun 1 Published in SASBDB: 2021 Oct 4

SASDJ34 – Human Complex I assembly factor ACAD9 dimer Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 130 kDa Homo sapiens protein Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2 RgGuinier 3.5 nm Dmax 13.0 nm VolumePorod 213 nm3

SASDJ44 – Human Complex I assembly factor ACAD9 complexed with ECSIT Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 130 kDa Homo sapiens protein Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial dimer, 93 kDa Homo sapiens protein Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2 RgGuinier 5.1 nm Dmax 16.8 nm VolumePorod 514 nm3

SASDJ54 – Human Complex I assembly factor ACAD9 complexed with ECSIT and SMT3-NDUFAF1 Sample: Complex I assembly factor ACAD9, mitochondrial tetramer, 259 kDa Homo sapiens protein Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial tetramer, 184 kDa Homo sapiens protein Complex I intermediate-associated protein 30, mitochondrial tetramer, 190 kDa Homo sapiens protein Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2 RgGuinier 6.6 nm Dmax 21.6 nm VolumePorod 1340 nm3