Tandem RNA binding sites induce self-association of the stress granule marker protein TIA-1.

Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA
Nucleic Acids Res (2021 Feb 24)

PMID: 33621982 doi: 10.1093/nar/gkab080

Submitted to SASBDB: 2020 Jul 14 Published in SASBDB: 2021 Feb 25

SASDJK5 – Nucleolysin TIA-1 isoform p40 (TIA-1) bound to TC1 DNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein TC1 monomer, 3 kDa synthetic construct DNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26 RgGuinier 3.2 nm Dmax 15.1 nm VolumePorod 78 nm3

SASDJL5 – Nucleolysin TIA-1 isoform p40 (TIA-1) bound to UC1 RNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein UC1 monomer, 3 kDa synthetic construct RNA Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26 RgGuinier 3.3 nm Dmax 14.4 nm VolumePorod 77 nm3

SASDJM5 – Modified nucleolysin TIA-1 isoform p40 (TIA-1 APO) Sample: Modified Nucleolysin TIA-1 isofrom p40 monomer, 42 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 60 mM KCl, 0.5 M arginine-HCl, 1 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, pH: 7 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2020 Jul 7 RgGuinier 3.2 nm Dmax 12.7 nm VolumePorod 62 nm3