The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection.

Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G
Nat Commun 12(1):1230 (2021 Feb 23)

PMID: 33623002 doi: 10.1038/s41467-021-21473-0

Submitted to SASBDB: 2020 Jul 16 Published in SASBDB: 2021 Jan 12

SASDK42 – Chitin-binding protein CbpD (Pseudomonas aeruginosa), ESRF BM29 data Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Oct 1 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 64131 nm3

SASDJQ5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 24 °C Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Jul 19 RgGuinier 3.3 nm Dmax 12.1 nm VolumePorod 50 nm3

SASDJR5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 37°C Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Aug 14 RgGuinier 3.5 nm Dmax 15.0 nm VolumePorod 63 nm3