Expression and analysis of the SAM-dependent RNA methyltransferase Rsm22 from Saccharomyces cerevisiae

Alam J, Rahman F, Sah-Teli S, Venkatesan R, Koski M, Autio K, Hiltunen J, Kastaniotis A
Acta Crystallographica Section D Structural Biology 77(6) (2021 May 19)

doi: 10.1107/S2059798321004149

Submitted to SASBDB: 2020 Jul 22 Published in SASBDB: 2021 May 25

SASDJS5 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer Sample: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1 RgGuinier 3.8 nm Dmax 13.9 nm VolumePorod 160 nm3

SASDJT5 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer Sample: Dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1 RgGuinier 5.0 nm Dmax 18.2 nm VolumePorod 516 nm3