Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA.

Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
Eur Biophys J (2021 Jan 4)

PMID: 33398461 doi: 10.1007/s00249-020-01489-y

Submitted to SASBDB: 2020 Jul 24 Published in SASBDB: 2021 Jan 8

SASDJ46 – Fep1-rec-GATA Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5 RgGuinier 3.5 nm Dmax 13.1 nm VolumePorod 73 nm3

SASDJ56 – Fep1-GATA Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 85 nm3

SASDJ66 – GATA double strand DNA Sample: 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5 RgGuinier 2.1 nm Dmax 7.4 nm VolumePorod 10 nm3

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5 RgGuinier 3.8 nm Dmax 16.5 nm VolumePorod 53 nm3

SASDJ86 – Fep1 wild type Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5 RgGuinier 3.5 nm Dmax 11.8 nm VolumePorod 41 nm3