Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly.

Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
J Mol Biol 432(21):5736-5751 (2020 Oct 2)
PMID: 32898582
doi: 10.1016/j.jmb.2020.08.023 		Submitted to SASBDB: 2021 Dec 13
Published in SASBDB:

SASDNU3 – Fibrillin PF2

Fibrillin-1 experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2006 Feb 11
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 84 nm3

SASDNV3 – Synthetic Human Elastin without domain 26A

Elastin experimental SAS data
Elastin Kratky plot
Sample: Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2011 Mar 15
RgGuinier 6.6 nm
Dmax 22.5 nm

SASDNW3 – Fibrillin-1 fragment PF2 crosslinked to tropoelastin by transglutaminase 2

Fibrillin-1Elastin experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 21
RgGuinier 7.1 nm
Dmax 23.5 nm