Structural Characterization of Plant Metallothionein

Dima Molodenskiy

Submitted to SASBDB: 2020 Aug 14 Published in SASBDB: 2022 Feb 2

SASDJE6 – Metallothionein (MT) 2 protein from the plant Cicer arietinum Sample: Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein Buffer: 10 mM Tris, 50 mM NaCl, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8 RgGuinier 1.9 nm Dmax 7.3 nm VolumePorod 5 nm3

SASDJF6 – Apo-full-length protein titrated with Cd(II) ions Sample: Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein Buffer: 10 mM Tris, 50 mM NaCl + 1mM TCEP (apoMT), pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8 RgGuinier 2.6 nm Dmax 10.0 nm VolumePorod 13 nm3

SASDJG6 – The separated apo-linker region peptide Sample: The separated apo-linker region peptide from the plant Cicer arietinum monomer, 4 kDa Cicer arietinum protein Buffer: 10 mM Tris, 50 mM NaCl, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8 RgGuinier 1.4 nm Dmax 6.5 nm VolumePorod 1 nm3

SASDJH6 – The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site Sample: A CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site monomer, 5 kDa Cicer arietinum protein Buffer: 10 mM Tris, 50 mM NaCl, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8 RgGuinier 1.9 nm Dmax 6.3 nm