SAXS Measurements of the SAM2 Riboswitch

Alex Plumridge

Submitted to SASBDB: 2020 Aug 19 Published in SASBDB: 2023 Aug 19

SASDJ67 – SAM2 Riboswitch 20 mM NaCl Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10 RgGuinier 3.5 nm

SASDJ77 – SAM2 Riboswitch 50 mM NaCl Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 50 mM NaCl, 20 μM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 3.3 nm

SASDJ87 – SAM2 Riboswitch 100 mM NaCl Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 100 mM NaCl, 20 μM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 3.0 nm

SASDJ97 – SAM2 Riboswitch 200 mM NaCl Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 200 mM NaCl, 20μM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 3.0 nm

SASDJA7 – SAM2 Riboswitch 0.5 mM MgCl2 Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.5 mM MgCl2, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 3.2 nm

SASDJB7 – SAM2 Riboswitch 1 mM MgCl2 Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1mM MgCl2, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 2.5 nm

SASDJC7 – SAM2 Riboswitch 2 mM MgCl2 Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 2.2 nm

SASDJD7 – SAM2 Riboswitch 3 mM MgCl2 Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 2.1 nm

SASDJE7 – SAM2 Riboswitch 3.75 mM MgCl2 Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 9 RgGuinier 2.1 nm

SASDJF7 – SAM2 Riboswitch 0.25 mM MgCl2 Saturating SAM Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.25 mM MgCl2 SAM, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10 RgGuinier 2.7 nm

SASDJG7 – SAM2 Riboswitch 1 mM MgCl2 Saturating SAM Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 1 mM MgCl2 SAM, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10 RgGuinier 2.0 nm

SASDJH7 – SAM2 Riboswitch 2 mM MgCl2 Saturating SAM Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2 SAM, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10 RgGuinier 2.0 nm

SASDJJ7 – SAM2 Riboswitch 3 mM MgCl2 Saturating SAM Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2 SAM, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10 RgGuinier 2.0 nm

SASDJK7 – SAM2 Riboswitch 3.75 mM MgCl2 Saturating SAM Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2 SAM, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10 RgGuinier 2.0 nm