Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD

Ostendorp A, Ostendorp S, Zhou Y, Chaudron Z, Wolffram L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
Journal of Biological Chemistry :102631 (2022 Oct)

doi: 10.1016/j.jbc.2022.102631 		Submitted to SASBDB: 2020 Sep 5
Published in SASBDB:

SASDJS7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 46 nm3

SASDJT7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 48 nm3