Notch-Jagged signaling complex defined by an interaction mosaic.

Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
Proc Natl Acad Sci U S A 118(30) (2021 Jul 27)

PMID: 34301900 doi: 10.1073/pnas.2102502118

Submitted to SASBDB: 2020 Sep 23 Published in SASBDB: 2021 Jun 30

SASDJ38 – Monomeric Jagged1 high-affinity full ectodomain Sample: Protein jagged-1 monomer, 115 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 12 RgGuinier 7.4 nm Dmax 24.0 nm VolumePorod 375 nm3

SASDJ48 – Jagged1 high-affinity full ectodomain 5.3 µM Sample: Protein jagged-1, 115 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 8.1 nm Dmax 30.0 nm VolumePorod 430 nm3

SASDJ58 – Jagged1 high-affinity full ectodomain 11 µM Sample: Protein jagged-1, 115 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 8.9 nm Dmax 33.0 nm VolumePorod 505 nm3

SASDJ68 – Jagged1 high-affinity full ectodomain 21 µM Sample: Protein jagged-1, 115 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 9.6 nm Dmax 42.0 nm VolumePorod 715 nm3

SASDJ78 – Jagged1 high-affinity full ectodomain 42 µM Sample: Protein jagged-1, 115 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 10.2 nm Dmax 43.0 nm VolumePorod 900 nm3

SASDJ88 – Jagged1 EGF8-11 29 µM Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 3.3 nm Dmax 11.0 nm VolumePorod 26 nm3

SASDJ98 – Jagged1 EGF8-11 58 µM Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 3.2 nm Dmax 11.5 nm VolumePorod 31 nm3

SASDJA8 – Jagged1 EGF8-11 115 µM Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 3.1 nm Dmax 11.5 nm VolumePorod 34 nm3

SASDJB8 – Jagged1 EGF8-11 230 µM Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 66 nm3

SASDJC8 – Jagged1 cysteine-rich domain (CRD) 21 µM Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 2.3 nm Dmax 7.5 nm VolumePorod 51 nm3

SASDJD8 – Jagged1 cysteine-rich domain (CRD) 42 µM Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 2.3 nm Dmax 7.8 nm VolumePorod 56 nm3

SASDJE8 – Jagged1 cysteine-rich domain (CRD) 83 µM Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 2.3 nm Dmax 8.2 nm VolumePorod 59 nm3

SASDJF8 – Jagged1 cysteine-rich domain (CRD) 167 µM Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11 RgGuinier 2.4 nm Dmax 9.0 nm VolumePorod 61 nm3

SASDJG8 – Monomeric neurogenic locus notch homolog protein 1 (Notch1) full ectodomain Sample: Neurogenic locus notch homolog protein 1 full ectodomain monomer, 183 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25 RgGuinier 10.5 nm Dmax 38.0 nm VolumePorod 1000 nm3