Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents.

Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
Biochemistry 60(14):1108-1119 (2021 Apr 13)
PMID: 33755420
doi: 10.1021/acs.biochem.0c00924 		Submitted to SASBDB: 2020 Oct 4
Published in SASBDB:

SASDJL9 – Saposin-A monomer

Prosaposin (Saposin A) experimental SAS data
Prosaposin (Saposin A) Kratky plot
Sample: Prosaposin (Saposin A) monomer, 10 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 1.5 nm
Dmax 5.0 nm
VolumePorod 23 nm3

SASDJM9 – Saposin-A plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.3 nm
Dmax 6.2 nm

SASDJN9 – Saposin-A plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.5 nm
Dmax 6.7 nm

SASDJP9 – Saposin-A plus LMNG (2,2-didecylpropane-1,3-bis-β-D-maltopyranoside)

Prosaposin (Saposin-A)2,2-didecylpropane-1,3-bis-β-D-maltopyranoside experimental SAS data
Prosaposin (Saposin-A) 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside 0, 15 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.5 nm
Dmax 7.0 nm

SASDJQ9 – Saposin-B, R232W mutant

Prosaposin (Saposin-B, R232W mutant) experimental SAS data
Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.0 nm
Dmax 5.8 nm

SASDJR9 – Saposin-B, R232W mutant, plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitolProsaposin (Saposin-B, R232W mutant) experimental SAS data
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.3 nm
Dmax 6.9 nm

SASDJS9 – Saposin-B, R232W mutant, plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitolProsaposin (Saposin-B, R232W mutant) experimental SAS data
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.6 nm
Dmax 7.7 nm

SASDJT9 – Saposin-B, R232W mutant, plus LMNG (2,2-didecylpropane-1,3-bis-β-D-maltopyranoside)

2,2-didecylpropane-1,3-bis-β-D-maltopyranosideProsaposin (Saposin-B, R232W mutant) experimental SAS data
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside 0, 15 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
RgGuinier 2.8 nm
Dmax 8.9 nm