Comparison of angiopoietin-like protein 3 and 4 reveals structural and mechanistic similarities.

Gunn KH, Gutgsell AR, Xu Y, Johnson CV, Liu J, Neher SB
J Biol Chem :100312 (2021 Jan 19)

PMID: 33482195 doi: 10.1016/j.jbc.2021.100312

Submitted to SASBDB: 2020 Sep 22 Published in SASBDB: 2021 Jan 14

SASDJK8 – N-terminal Angiopoietin-like protein 3 Hexamer Sample: Angiopoietin-like protein 3 (N-terminal) hexamer, 161 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl pH 7.5, 400 mM NaCl, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12 RgGuinier 5.6 nm Dmax 34.0 nm VolumePorod 380 nm3

SASDJL8 – N-terminal Angiopoietin-like protein 3 Trimer Sample: Angiopoietin-related protein 3 trimer, 81 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl pH 7.5, 400 mM NaCl, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12 RgGuinier 4.6 nm Dmax 21.5 nm VolumePorod 71 nm3

SASDJM8 – N-terminal Angiopoietin-like protein 4 Trimer Sample: Angiopoietin-related protein 4 trimer, 46 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl pH 7.4, 300 mM NaCl, 100 mM betaine, 500 mM arginine, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 8 RgGuinier 4.7 nm Dmax 16.0 nm VolumePorod 69 nm3