Inference of molecular structure for characterization and improvement of clinical grade immunocytokines

Ongaro T, Guarino S, Scietti L, Palamini M, Wulhfard S, Neri D, Villa A, Forneris F
Journal of Structural Biology :107696 (2021 Jan)

doi: 10.1016/j.jsb.2021.107696

Submitted to SASBDB: 2020 Sep 24 Published in SASBDB: 2021 Jan 26

SASDJN8 – L19-IL2 dimeric immunocytokine Sample: L19-IL2 dimeric immunocytokine dimer, 84 kDa synthetic construct protein Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 23 RgGuinier 4.1 nm Dmax 11.9 nm VolumePorod 128 nm3

SASDJP8 – L19L19-IL2 immunocytokine monomer Sample: L19L19-IL2 immunocytokine monomer, 69 kDa synthetic construct protein Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 4 RgGuinier 3.6 nm Dmax 10.8 nm VolumePorod 93 nm3

SASDJQ8 – IL12-L19L19 immunocytokine monomer Sample: IL12-L19L19 immunocytokine monomer, 112 kDa synthetic construct protein Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 4 RgGuinier 4.5 nm Dmax 14.2 nm VolumePorod 224 nm3