Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations

Nguyen G, Sutinen A, Raasakka A, Muruganandam G, Loris R, Kursula P
Frontiers in Molecular Biosciences 7 (2021 Jan 27)

doi: 10.3389/fmolb.2020.631232 		Submitted to SASBDB: 2020 Oct 8
Published in SASBDB:

SASDJR8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 dimer, 68 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 21
RgGuinier 3.1 nm
Dmax 101.6 nm
VolumePorod 111 nm3

SASDJS8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 with hexadecanedioic acid

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 dimer, 68 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
RgGuinier 3.1 nm
Dmax 9.2 nm
VolumePorod 112 nm3

SASDJT8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 21
RgGuinier 2.7 nm
Dmax 92.3 nm
VolumePorod 71 nm3

SASDJU8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 with hexadecanedioic acid

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 63 nm3

SASDJV8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358 experimental SAS data
CORAL model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358 dimer, 70 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jun 28
RgGuinier 3.1 nm
Dmax 9.9 nm
VolumePorod 106 nm3

SASDJW8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A monomer, 35 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jun 28
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 59 nm3

SASDJX8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
RgGuinier 3.5 nm
Dmax 10.8 nm
VolumePorod 130 nm3

SASDJY8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
RgGuinier 3.1 nm
Dmax 9.6 nm
VolumePorod 121 nm3

SASDJZ8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
RgGuinier 2.7 nm
Dmax 9.0 nm
VolumePorod 69 nm3

SASDJ29 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
RgGuinier 2.5 nm
Dmax 8.6 nm
VolumePorod 65 nm3

SASDJ39 – Monomeric human ganglioside-induced differentiation-associated protein 1-like 1, GDAP1L1

Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) experimental SAS data
CORAL model
Sample: Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM TRIS pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 10
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 73 nm3