Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis
Asthana P,
Singh D,
Pedersen J,
Hynönen M,
Sulu R,
Murthy A,
Laitaoja M,
Jänis J,
Riley L,
Venkatesan R
IUCrJ
8(5)
(2021 Jul 28)
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Sample: |
Mce-family protein Mce1A monomer monomer, 17 kDa Mycobacterium tuberculosis protein
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Buffer: |
50 mM Tris, 350 mM NaCl, 10% Glycerol, pH: 8.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 May 10
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RgGuinier |
2.1 |
nm |
Dmax |
8.7 |
nm |
VolumePorod |
29 |
nm3 |
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Sample: |
Mce-family protein Mce1A monomer, 48 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 112 kDa
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Buffer: |
50 mM Tris, 500 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 8 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2018 May 13
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RgGuinier |
5.3 |
nm |
Dmax |
21.6 |
nm |
VolumePorod |
362 |
nm3 |
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Sample: |
Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
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Buffer: |
50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2018 Nov 28
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RgGuinier |
5.5 |
nm |
Dmax |
22.0 |
nm |
VolumePorod |
216 |
nm3 |
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Sample: |
Mce-family protein Mce1A monomer, 36 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 82 kDa
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Buffer: |
50 mM Tris, 500 mM NaCl, 10% Glycerol, 5 mM DDM, 1 mM β-ME, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 May 2
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RgGuinier |
4.6 |
nm |
Dmax |
17.5 |
nm |
VolumePorod |
151 |
nm3 |
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Sample: |
Mce-family protein Mce4A monomer, 15 kDa Mycobacterium tuberculosis (strain … protein
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Buffer: |
50mM MOPS, 350mM NaCl, 10% Glycerol, pH: 7 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 May 10
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|
RgGuinier |
2.1 |
nm |
Dmax |
7.8 |
nm |
VolumePorod |
22 |
nm3 |
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Sample: |
Mce-family protein Mce4A monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
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Buffer: |
50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2018 May 13
|
|
RgGuinier |
5.7 |
nm |
Dmax |
21.5 |
nm |
VolumePorod |
446 |
nm3 |
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Sample: |
N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 35 kDa Mycobacterium tuberculosis (strain … protein
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Buffer: |
50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2018 Nov 28
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RgGuinier |
5.0 |
nm |
Dmax |
19.1 |
nm |
VolumePorod |
278 |
nm3 |
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Sample: |
N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
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Buffer: |
50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 May 2
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RgGuinier |
4.8 |
nm |
Dmax |
18.2 |
nm |
VolumePorod |
189 |
nm3 |
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