Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins.

Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
Biochem Biophys Res Commun 550:43-48 (2021 Apr 23)

PMID: 33684619 doi: 10.1016/j.bbrc.2021.02.114

Submitted to SASBDB: 2020 Oct 27 Published in SASBDB: 2021 Apr 6

SASDKA2 – Two titin I27 domains linked with RS linker Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4 RgGuinier 3.0 nm

SASDKB2 – Two titin I27 domains linked with RS linker Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4 RgGuinier 3.0 nm

SASDKC2 – Two titin I27 domains linked with GGG linker Sample: Two titin I27 domains linked with GGG linker monomer, 23 kDa protein Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4 RgGuinier 2.9 nm

SASDKD2 – Two titin I27 domains linked with VKA linker Sample: Two titin I27 domains linked with VKA linker monomer, 24 kDa protein Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4 RgGuinier 3.1 nm

SASDKE2 – Two titin I27 domains linked with AAE linker Sample: Two titin I27 domains linked with AAE linker monomer, 24 kDa protein Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4 RgGuinier 3.1 nm