High Conformational Flexibility of the E2F1/DP1/DNA Complex.

Saad D, Paissoni C, Chaves-Sanjuan A, Nardini M, Mantovani R, Gnesutta N, Camilloni C
J Mol Biol 433(18):167119 (2021 Jun 25)
PMID: 34181981
doi: 10.1016/j.jmb.2021.167119 		Submitted to SASBDB: 2020 Oct 29
Published in SASBDB:

SASDKH2 – E2F1/DP1 in complex with a 15-mer DNA oligo

Transcription factor E2F115-mer DNA oligo of the human E2F1 promoterE2F dimerization partner 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transcription factor E2F1 monomer, 20 kDa Homo sapiens protein
15-mer DNA oligo of the human E2F1 promoter monomer, 9 kDa DNA
E2F dimerization partner 1 monomer, 29 kDa Homo sapiens protein
Buffer: 50 mM Tris-Cl pH 8.0, 150 mM NaCl and 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 19
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 99 nm3