Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation.

Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I
Commun Biol 4(1):600 (2021 May 20)

PMID: 34017052 doi: 10.1038/s42003-021-02132-y

Submitted to SASBDB: 2020 Nov 17 Published in SASBDB: 2021 May 11

SASDK24 – Signal recognition particle SRP9/14 heterodimer from Plasmodium falciparum Sample: Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 22 RgGuinier 2.1 nm Dmax 7.2 nm VolumePorod 47 nm3

SASDK34 – Full length SRP Alu RNA from Plasmodium falciparum Sample: Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 22 RgGuinier 3.3 nm Dmax 11.8 nm VolumePorod 63 nm3

SASDK44 – The 5' domain of SRP Alu RNA from Plasmodium falciparum Sample: SRP Alu RNA 5' domain monomer, 24 kDa Plasmodium falciparum RNA Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 22 RgGuinier 3.3 nm Dmax 11.5 nm VolumePorod 38 nm3

SASDK54 – Signal recognition particle SRP9/14 heterodimer in complex with full length SRP Alu RNA from Plasmodium falciparum Sample: Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 22 RgGuinier 3.5 nm Dmax 12.0 nm VolumePorod 120 nm3

SASDK64 – Signal recognition particle SRP9/14 heterodimer in complex with the 5' domain of SRP Alu RNA from Plasmodium falciparum Sample: Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein SRP Alu RNA 5' domain monomer, 24 kDa Plasmodium falciparum RNA Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 22 RgGuinier 3.2 nm Dmax 11.9 nm VolumePorod 77 nm3