BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture.

Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G
Nucleic Acids Res (2021 Aug 25)

PMID: 34432045 doi: 10.1093/nar/gkab736

Submitted to SASBDB: 2020 Nov 19 Published in SASBDB: 2021 Aug 16

SASDK74 – Streptococcus agalactiae transcription factor BusR - RCK_C domain Sample: Transcriptional repressor BusR RCK_C domain dimer, 22 kDa Streptococcus agalactiae serotype … protein Buffer: 100 mM NaCl, 30mM Hepes, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2 RgGuinier 1.9 nm Dmax 6.4 nm VolumePorod 44 nm3

SASDK84 – Streptococcus agalactiae transcription factor BusR Sample: Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein Buffer: 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2 RgGuinier 4.4 nm Dmax 13.9 nm VolumePorod 168 nm3

SASDK94 – Streptococcus agalactiae transcription factor BusR dsDNA complex Sample: Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein BusR Recognition sequence monomer, 28 kDa synthetic construct DNA Buffer: 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2 RgGuinier 4.3 nm Dmax 14.2 nm VolumePorod 210 nm3