Despite the odds: formation of the SARS-CoV-2 methylation complex

Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A
Nucleic Acids Research (2024 Mar 18)

doi: 10.1093/nar/gkae165

Submitted to SASBDB: 2020 Dec 27 Published in SASBDB: 2023 Aug 10

SASDKT6 – SARS-CoV-2 non-structural protein 14 (nsp14) Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11 RgGuinier 2.7 nm Dmax 9.6 nm VolumePorod 80 nm3

SASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 ) Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11 RgGuinier 2.9 nm Dmax 12.2 nm VolumePorod 73 nm3

SASDKV6 – SARS-CoV-2 non-structural protein 10/non-structural protein 16 complex (nsp10/nsp16) Sample: Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11 RgGuinier 2.0 nm Dmax 8.0 nm VolumePorod 28 nm3

SASDKW6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16) Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11 RgGuinier 4.7 nm Dmax 11.4 nm VolumePorod 113 nm3