Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR

Toyama Y, Harkness R, Lee T, Maynes J, Kay L
Proceedings of the National Academy of Sciences 118(11):e2025022118 (2021 Mar 10)

doi: 10.1073/pnas.2025022118

Submitted to SASBDB: 2020 Dec 4 Published in SASBDB: 2021 Mar 5

SASDKL4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 10 mg/ml) Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1 RgGuinier 4.2 nm Dmax 16.0 nm VolumePorod 250 nm3

SASDKM4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 5 mg/ml) Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1 RgGuinier 3.8 nm Dmax 15.0 nm VolumePorod 213 nm3

SASDKN4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 2 mg/ml) Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1 RgGuinier 3.6 nm Dmax 12.8 nm VolumePorod 203 nm3

SASDKP4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 1 mg/ml) Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1 RgGuinier 3.4 nm Dmax 12.0 nm VolumePorod 168 nm3