Teneurin4 dimer structures reveal a calcium‐stabilized compact conformation supporting homomeric trans‐interactions

Meijer D, Frias C, Beugelink J, Deurloo Y, Janssen B
The EMBO Journal (2022 Jan 31)

doi: 10.15252/embj.2020107505 		Submitted to SASBDB: 2020 Dec 15
Published in SASBDB:

SASDKS4 – Teneurin-4 dimer wildtype in SEC buffer (20 mM HEPES, 150 mM NaCl)

Teneurin-4 experimental SAS data
Teneurin-4 dimer wildtype in SEC buffer (20 mM HEPES, 150 mM NaCl) Rg histogram
Sample: Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 7.8 nm
Dmax 32.0 nm
VolumePorod 1280 nm3

SASDKT4 – Teneurin-4 dimer wildtype in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2)

Teneurin-4 experimental SAS data
Teneurin-4 dimer wildtype in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2) Rg histogram
Sample: Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 7.2 nm
Dmax 29.5 nm
VolumePorod 1270 nm3

SASDKU4 – Teneurin-4 dimer wildtype in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA)

Teneurin-4 experimental SAS data
Teneurin-4 dimer wildtype in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA) Rg histogram
Sample: Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM EDTA, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 9.5 nm
Dmax 40.0 nm
VolumePorod 1370 nm3

SASDKV4 – Teneurin-4 dimer mutant in SEC buffer (20 mM HEPES, 150 mM NaCl)

Teneurin-4 (S2585C) experimental SAS data
Teneurin-4 dimer mutant in SEC buffer (20 mM HEPES, 150 mM NaCl) Rg histogram
Sample: Teneurin-4 (S2585C) dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 7.1 nm
Dmax 29.5 nm
VolumePorod 1260 nm3

SASDKW4 – Teneurin-4 dimer mutant in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2)

Teneurin-4 (S2585C) experimental SAS data
Teneurin-4 dimer mutant in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2) Rg histogram
Sample: Teneurin-4 (S2585C) dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 7.0 nm
Dmax 29.5 nm
VolumePorod 1270 nm3

SASDKX4 – Teneurin-4 dimer mutant in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA)

Teneurin-4 (S2585C) experimental SAS data
Teneurin-4 dimer mutant in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA) Rg histogram
Sample: Teneurin-4 (S2585C) dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM EDTA, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
RgGuinier 7.8 nm
Dmax 31.0 nm
VolumePorod 1390 nm3