Identification and structural analysis of the Schizosaccharomyces pombe SMN complex

Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
Nucleic Acids Research (2021 Mar 23)

doi: 10.1093/nar/gkab158 		Submitted to SASBDB: 2020 Dec 17
Published in SASBDB:

SASDKZ4 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.4 nm
Dmax 29.0 nm

SASDK25 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.165 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.3 nm
Dmax 32.0 nm

SASDK35 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.419 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.2 nm
Dmax 29.7 nm

SASDK45 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.879 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.1 nm
Dmax 29.0 nm

SASDK55 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.422 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.0 nm
Dmax 33.0 nm

SASDK65 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.185 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.1 nm
Dmax 31.0 nm

SASDK75 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.107 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 7.1 nm
Dmax 30.5 nm

SASDK85 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 7.9 nm
Dmax 27.0 nm

SASDK95 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.330 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.0 nm
Dmax 29.0 nm

SASDKA5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.700 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 8.0 nm
Dmax 27.3 nm

SASDKB5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 1.47 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 7.9 nm
Dmax 28.0 nm

SASDKC5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.711 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 7.6 nm
Dmax 27.0 nm

SASDKD5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.373 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 7.5 nm
Dmax 26.0 nm

SASDKE5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.173 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Gem-associated protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 6.3 nm
Dmax 21.3 nm

SASDKF5 – the Gemin2/delta linker Survival Motor Neuron-like protein 1 (SMN1, Δ36-119, S130D) complex from Schizosaccharomyces pombe

Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80)Survival motor neuron-like protein 1 (Δ36-119) experimental SAS data
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Survival motor neuron-like protein 1 (Δ36-119) Kratky plot
Sample: Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Survival motor neuron-like protein 1 (Δ36-119) monomer, 8 kDa Schizosaccharomyces pombe protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30
RgGuinier 2.7 nm
Dmax 9.0 nm

SASDK66 – the Gemin2/delta linker Survival Motor Neuron-like protein 1 (SMN1, Δ36-119) complex from Schizosaccharomyces pombe

Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80)Survival motor neuron-like protein 1 (Δ36-119) experimental SAS data
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Survival motor neuron-like protein 1 (Δ36-119) Kratky plot
Sample: Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Survival motor neuron-like protein 1 (Δ36-119) monomer, 8 kDa Schizosaccharomyces pombe protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 386 nm3