Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids

Yen-Lin Chen Submitted to SASBDB: 2020 Dec 15
Published in SASBDB:

SASDKG5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKH5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKJ5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKK5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKL5 – RNA Short Tetraloop Hairpin Triplex

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RgGuinier 2.0 nm
Dmax 6.3 nm

SASDKM5 – Metastasis-Associated Lung Adenocarcinoma Transcript 1

Metastasis-Associated Lung Adenocarcinoma Transcript 1 experimental SAS data
Metastasis-Associated Lung Adenocarcinoma Transcript 1 Kratky plot
Sample: Metastasis-Associated Lung Adenocarcinoma Transcript 1 monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 μM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
RgGuinier 2.3 nm
Dmax 7.9 nm

SASDKN5 – 30nt Single-stranded RNA

30nt Single-stranded RNA experimental SAS data
30nt Single-stranded RNA Kratky plot
Sample: 30nt Single-stranded RNA monomer, 10 kDa RNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RgGuinier 2.2 nm
Dmax 8.2 nm