The Induction Mechanism of the Flavonoid-Responsive Regulator FrrA.

Werner N, Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
FEBS J (2021 Jul 27)

PMID: 34314575 doi: 10.1111/febs.16141

Submitted to SASBDB: 2020 Dec 19 Published in SASBDB: 2021 Aug 1

SASDKA6 – Flavonoid-responsive regulator A (FrrA) Sample: TetR/AcrR family transcriptional regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23 RgGuinier 3.4 nm Dmax 9.0 nm VolumePorod 93 nm3

SASDKB6 – Flavonoid-responsive regulator A (FrrA) in the presence of genistein Sample: TetR/AcrR family transcriptional regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein 5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one monomer, 0 kDa Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, 0.08 mM genistein, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 27 RgGuinier 3.4 nm Dmax 8.7 nm VolumePorod 96 nm3

SASDKC6 – Flavonoid-responsive regulator A (FrrA) in the presence of naringenin Sample: TetR/AcrR family transcriptional regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein 5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one monomer, 0 kDa Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, 1 mM naringenin, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23 RgGuinier 3.2 nm Dmax 8.6 nm VolumePorod 91 nm3