Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase

Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
Journal of Structural Biology 213(2):107724 (2021 Jun)

doi: 10.1016/j.jsb.2021.107724

Submitted to SASBDB: 2020 Dec 18 Published in SASBDB: 2021 Jan 2

SASDKH6 – Sulfite reductase flavoprotein-43/hemoprotein heterodimer Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 42 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11 RgGuinier 3.2 nm Dmax 9.9 nm

SASDKJ6 – Sulfite reductase flavoprotein-60/hemoprotein heterodimer Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11 RgGuinier 3.7 nm Dmax 13.4 nm

SASDKK6 – Deuterated sulfite reductase hemoprotein Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 May 28 RgGuinier 2.6 nm Dmax 7.5 nm

SASDKL6 – Sulfite reductase flavoprotein-60 reduced with 10 molar equivalents sodium dithionite Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11 RgGuinier 3.4 nm Dmax 12.2 nm

SASDKM6 – Sulfite reductase flavoprotein-60 (contrast-matched)/deuterated-hemoprotein heterodimer Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 Jun 28 RgGuinier 2.4 nm Dmax 6.6 nm

SASDKN6 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein (contrast-matched) heterodimer Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8 Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 Jun 26 RgGuinier 3.2 nm Dmax 12.4 nm