The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites

Benavente J, Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A
Life Science Alliance 4(10):e202101152 (2021 Aug 18)

doi: 10.26508/lsa.202101152

Submitted to SASBDB: 2020 Dec 22 Published in SASBDB: 2021 Aug 6

SASDKF6 – C2AB construct of synaptotagmin-1 (SYT1) with E395-397A mutations at the linker Sample: Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein Buffer: 50 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 11 RgGuinier 3.1 nm Dmax 12.1 nm VolumePorod 59 nm3

SASDKG6 – SMP2C2A construct of synaptotagmin-1 (SYT1) Sample: Synaptotagmin-1 (SYT1-SMP2C2A) monomer, 72 kDa Escherichia coli protein Buffer: 20 mM Tris, 100 mM NaCl, 5% glycerol, 2 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 11 RgGuinier 4.2 nm Dmax 17.8 nm VolumePorod 138 nm3

SASDKJ9 – C2AB construct of synaptotagmin-1 (SYT1) Sample: Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein Buffer: 50 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 16 RgGuinier 2.8 nm Dmax 12.6 nm VolumePorod 50 nm3

SASDKK9 – C2AB construct of synaptotagmin-1 (SYT1) in presence of Ca cations Sample: Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein Buffer: 50 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 16 RgGuinier 2.9 nm Dmax 13.8 nm VolumePorod 54 nm3