Nucleosome scaffolding by Brd4 tandem bromodomains in acetylation-dependent chromatin compartmentalization

Olp M, Jackson V, Smith B
(2019 Jul 11)

doi: 10.1101/699967

Submitted to SASBDB: 2020 Dec 31 Published in SASBDB: 2023 Dec 31

SASDKH7 – Bromodomain testis-specific protein, BRDT, tandem bromodomains (10 mg/ml) Sample: Bromodomain testis-specific protein monomer, 42 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 5.7 nm Dmax 18.6 nm VolumePorod 120 nm3

SASDKJ7 – Bromodomain testis-specific protein, BRDT, tandem bromodomains (5 mg/ml) Sample: Bromodomain testis-specific protein monomer, 42 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 5.0 nm Dmax 19.2 nm VolumePorod 120 nm3

SASDK77 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (10 mg/ml) Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 5.2 nm Dmax 18.3 nm VolumePorod 100 nm3

SASDK87 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (5 mg/ml) Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 4.9 nm Dmax 18.5 nm VolumePorod 100 nm3

SASDK97 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (2 mg/ml) Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 4.6 nm Dmax 17.0 nm VolumePorod 100 nm3

SASDKA7 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (1 mg/ml) Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 4.7 nm Dmax 18.8 nm VolumePorod 80 nm3

SASDKB7 – Bromodomain-containing protein 3, BRD3, tandem bromodomains (10 mg/ml) Sample: Bromodomain-containing protein 3 monomer, 44 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 5.2 nm Dmax 18.6 nm VolumePorod 100 nm3

SASDKC7 – Bromodomain-containing protein 3, BRD3, tandem bromodomains (2 mg/ml) Sample: Bromodomain-containing protein 3 monomer, 44 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 4.5 nm Dmax 18.7 nm VolumePorod 90 nm3

SASDKD7 – Bromodomain-containing protein 3, BRD3, tandem bromodomains (1 mg/ml) Sample: Bromodomain-containing protein 3 monomer, 44 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 4.7 nm Dmax 17.8 nm VolumePorod 85 nm3

SASDKE7 – Bromodomain-containing protein 4, BRD4, tandem bromodomains (10 mg/ml) Sample: Bromodomain-containing protein 4 monomer, 48 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 7.3 nm Dmax 20.2 nm VolumePorod 150 nm3

SASDKF7 – Bromodomain-containing protein 4, BRD4, tandem bromodomains (5 mg/ml) Sample: Bromodomain-containing protein 4 monomer, 48 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 5.5 nm Dmax 20.2 nm VolumePorod 145 nm3

SASDKG7 – Bromodomain-containing protein 4, BRD4, tandem bromodomains (2 mg/ml) Sample: Bromodomain-containing protein 4 monomer, 48 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 5.2 nm Dmax 20.1 nm VolumePorod 100 nm3

SASDKK7 – Transcription initiation factor TFIID subunit 1, TAF1, tandem bromodomains (2 mg/ml) Sample: Transcription initiation factor TFIID subunit 1 monomer, 31 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 3.2 nm Dmax 9.9 nm VolumePorod 80 nm3

SASDKL7 – Transcription initiation factor TFIID subunit 1, TAF1, tandem bromodomains (1 mg/ml) Sample: Transcription initiation factor TFIID subunit 1 monomer, 31 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25 RgGuinier 2.9 nm Dmax 10.6 nm VolumePorod 65 nm3