The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection.

Vieira MFM, Hernandez G, Zhong Q, Arbesú M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazão C, Frankel G, Zanzoni A, Cordeiro TN
Commun Biol 7(1):179 (2024 Feb 13)

PMID: 38351154 doi: 10.1038/s42003-024-05856-9

Submitted to SASBDB: 2021 Feb 12 Published in SASBDB: 2021 Nov 29

SASDKF8 – N-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor Sample: Translocated intimin receptor Tir dimer, 50 kDa Escherichia coli O127:H6 … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 18 RgGuinier 3.5 nm Dmax 14.0 nm VolumePorod 46 nm3

SASDKG8 – Central domain of the N-terminal Region of the Translocated Intimin Receptor (60-200) Sample: Translocated intimin receptor Tir dimer, 32 kDa Escherichia coli O127:H6 … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 18 RgGuinier 3.4 nm Dmax 13.0 nm VolumePorod 47 nm3

SASDRQ5 – Small beta-barrel domain in the N-terminal region of the Translocated Intimin Receptor (Tir76-180) Sample: Translocated intimin receptor Tir monomer, 12 kDa Escherichia coli O127:H6 … protein Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Nov 21 RgGuinier 1.7 nm Dmax 7.0 nm VolumePorod 21 nm3

SASDKH8 – C-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor Sample: Translocated intimin receptor Tir monomer, 18 kDa Escherichia coli O127:H6 … protein Buffer: 20 mM Sodium Phosphate, 150 mM NaCl, 1 mM EDTA, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 15 RgGuinier 3.8 nm Dmax 12.8 nm VolumePorod 46 nm3