Substrate Induced Movement of the Metal Cofactor between Active and Resting State

Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
Angewandte Chemie International Edition (2022 Oct 10)

doi: 10.1002/anie.202213338

Submitted to SASBDB: 2021 Nov 3 Published in SASBDB: 2022 Oct 14

SASDKS7 – Hydroxypyruvate aldolase (HpcH/HpaI aldolase), wild-type in HEPES, NaCl and MgCl2 Sample: HpcH/HpaI aldolase hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein Buffer: 20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15 RgGuinier 3.3 nm Dmax 9.0 nm VolumePorod 224 nm3

SASDKT7 – Hydroxypyruvate aldolase (HpcH/HpaI aldolase), S116A mutant in HEPES, NaCl and MgCl2 Sample: HpcH/HpaI aldolase (S116A) hexamer, 165 kDa Sphingomonas wittichii (strain … protein Buffer: 20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15 RgGuinier 3.3 nm Dmax 9.1 nm VolumePorod 222 nm3

SASDKU7 – Hydroxypyruvate aldolase (HpcH/HpaI aldolase), S116C mutant in HEPES, NaCl and MgCl2 Sample: HpcH/HpaI aldolase (S116C) hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein Buffer: 20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15 RgGuinier 3.3 nm Dmax 9.0 nm VolumePorod 223 nm3