Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites.

Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
Structure 24(6):851-61 (2016 Jun 7)

PMID: 27133022 doi: 10.1016/j.str.2016.03.020

Submitted to SASBDB: 2015 Sep 24 Published in SASBDB: 2015 Sep 24

SASDB52 – Death associated protein kinase (wild-type) Sample: Death associated protein kinase wild-type, 37 kDa Homo sapiens protein Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18 RgGuinier 2.7 nm Dmax 8.1 nm VolumePorod 101 nm3

SASDB62 – Death associated protein kinase (D220K mutant) Sample: Death associated protein kinase (D220K mutant), 37 kDa Homo sapiens protein Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18 RgGuinier 2.5 nm Dmax 9.0 nm VolumePorod 89 nm3

SASDB72 – Death associated protein kinase (Basic Loop mutant) Sample: Death associated protein kinase (Basic Loop mutant), 37 kDa Homo sapiens protein Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18 RgGuinier 2.4 nm Dmax 7.5 nm VolumePorod 78 nm3