The molecular dissection of TRIM25's RNA-binding mechanism provides key insights into its antiviral activity.

Álvarez L, Haubrich K, Iselin L, Gillioz L, Ruscica V, Lapouge K, Augsten S, Huppertz I, Choudhury NR, Simon B, Masiewicz P, Lethier M, Cusack S, Rittinger K, Gabel F, Leitner A, Michlewski G, Hentze MW, Allain FHT, Castello A, Hennig J
Nat Commun 15(1):8485 (2024 Oct 1)

PMID: 39353916 doi: 10.1038/s41467-024-52918-x

Submitted to SASBDB: 2021 Jan 28 Published in SASBDB: 2021 Nov 24

SASDK78 – E3 ubiquitin/ISG15 ligase TRIM25, apo form (TRIM25 apo) Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 19 RgGuinier 6.8 nm Dmax 30.2 nm

SASDK88 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7) Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Pre-let-7-a-1@1 monomer, 9 kDa synthetic construct RNA Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 14 RgGuinier 5.7 nm Dmax 19.8 nm

SASDK98 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7): SEC-SAXS Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Pre-let-7-a-1@1 monomer, 9 kDa synthetic construct RNA Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 4 RgGuinier 5.7 nm Dmax 23.0 nm

SASDKA8 – E3 ubiquitin/ISG15 ligase TRIM25 bound to lnczc3h7a_304-326 RNA (TRIM25/lnczc3h7a) Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein Lnczc3h7a_304-326 monomer, 8 kDa Homo sapiens RNA Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 12 RgGuinier 5.8 nm Dmax 16.6 nm